Combinatorial fluorescent labeling of live anaerobic bacteria via …
WEBOct 11, 2023 · Combinatorial click chemistry labeling (COMBICK) enables labeling of multiple bacteria simultaneously, and is applicable to bacteria from across phylogenetic origins (including genetically ...
Using click chemistry to study microbial ecology and evolution
WEBJan 31, 2023 · Bioorthogonal chemistry, also known as click chemistry, is an approach in which a biomolecule is labelled with a small chemical group that can be subsequently ligated to a detectable group.
Recent Progress in Identifying Bacteria with Fluorescent Probes
WEBSep 29, 2022 · In the direct labeling method, the fluorescent probes are constructed with fluorophore-modified biological orthogonal precursors and incubated with bacteria. The fluorescent probes are directly labeled on the surface of bacteria through the unique metabolic pathway.
Strategies of Detecting Bacteria Using Fluorescence-Based Dyes
WEBAug 12, 2021 · The strategy for fluorescent probes targeting bacteria is as follows: 1) detect bacterial membranes based on interactions with cell wall components such as lipopolysaccharide (LPS); 2) detect bacterial enzymes such as nitroreductase (NTR), alkaline phosphatase (ALP), β-lactamases, and caspase-1; 3) label chemically fluorescent bacteria using ...
Click-electrochemistry for the rapid labeling of virus, bacteria and ...
WEBAug 23, 2023 · Here, we used N-methylluminol (NML), a fully tyrosine-selective protein anchoring group after one-electron oxidation, to label the surface of viruses, living bacteria and cells.
Fluorescent carbon dots for labeling of bacteria: mechanism and ...
WEBApr 24, 2024 · Among various fluorescent nanomaterials, fluorescent carbon dots (CDs) have emerged as a favorable staining agent for bacterial labeling due to their superior biocompatibility and biosafety, ease of preparation, and …
Combinatorial Click Chemistry Labeling to Study Live Human Gut …
WEBOct 27, 2021 · Here, we developed COMBInatorial cliCK-chemistry (COMBICK) labeling on human gut-derived bacteria, both aerobic and anaerobic strains, to enable dynamic tracing of live, heterogeneous bacterial communities on the strain level, including clinical isolates of the Enterobacteriaceae family.
Recent Progress in Identifying Bacteria with Fluorescent Probes
WEBSep 29, 2022 · Pathogenic bacterial identification strategies using fluorescent probes. 2. Metabolic Labeling of the Surface Components of Pathogenic Bacteria. Metabolic labeling is a technique for the incorporation of chemical molecules into macromolecules of interest via the intracellular enzyme.
Frontiers | A simple and efficient fluorescent labeling method in ...
WEBFeb 9, 2023 · Bacterial fluorescent labeling is a powerful tool for the diagnosis and treatment of bacterial infections. Here, we present a simple and efficient labeling strategy for Staphylococcus aureus. Intracellular labeling of bacteria was achieved by heat shock using Cyanine 5.5 (Cy5.5) near-infrared-I dyes in S. aureus (Cy5.5@S. aureus).
Bacterial enzymes: powerful tools for protein labeling, cell …
WEBJun 14, 2023 · Here we have discussed a series of bacterial enzymes that have been exploited for protein site-specific labeling in vitro and in vivo, proximity labeling, to elucidate biological pathways and for therapeutic applications.