In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Here, authors report the structure of the augmin’s CH domain bound to the microtubule lattice and show that its Haus8 subunit binds tubulin tails. Both domains are critical for microtubule binding and ...